CondSCVI is a variational inference model for single-cell RNA-seq data that can learn an underlying
latent space. The predictions of the model are meant to be afterward
used for deconvolution of a second spatial transcriptomics dataset in DestVI. DestVI predicts the
cell-type proportions as well as cell type-specific activation state
in the spatial data.
CondSCVI takes as input a scRNA-seq gene expression matrix with cells and genes as well as a
cell-type annotation for all cells.
We provide an extensive
user guide
for DestVI including a description of CondSCVI.
- See our original manuscript for further details of the model:
DestVI manuscript.
- See our manuscript on scvi-hub
how to leverage pre-trained models.
Model Description
Tabula Sapiens is a benchmark, first-draft human cell atlas of nearly 500,000 cells from 24 organs of 15 normal human subjects.
Metrics
We provide here key performance metrics for the uploaded model, if provided by the data uploader.
Coefficient of variation
The cell-wise coefficient of variation summarizes how well variation between different cells is
preserved by the generated model expression. Below a squared Pearson correlation coefficient of 0.4
, we would recommend not to use generated data for downstream analysis, while the generated latent
space might still be useful for analysis.
Cell-wise Coefficient of Variation:
Not provided by uploader
The gene-wise coefficient of variation summarizes how well variation between different genes is
preserved by the generated model expression. This value is usually quite high.
Gene-wise Coefficient of Variation:
Not provided by uploader
Differential expression metric
The differential expression metric provides a summary of the differential expression analysis
between cell types or input clusters. We provide here the F1-score, Pearson Correlation
Coefficient of Log-Foldchanges, Spearman Correlation Coefficient, and Area Under the Precision
Recall Curve (AUPRC) for the differential expression analysis using Wilcoxon Rank Sum test for each
cell-type.
Differential expression:
Not provided by uploader
Model Properties
We provide here key parameters used to setup and train the model.
Model Parameters
These provide the settings to setup the original model:
1{
2 "n_hidden": 128,
3 "n_latent": 5,
4 "n_layers": 2,
5 "weight_obs": false,
6 "dropout_rate": 0.05
7}
Setup Data Arguments
Arguments passed to setup_anndata of the original model:
1{
2 "batch_key": null,
3 "labels_key": "cell_type",
4 "fine_labels_key": null,
5 "layer": "counts",
6 "unlabeled_category": "unlabeled",
7 "size_factor_key": null
8}
Data Registry
Registry elements for AnnData manager:
| Registry Key | scvi-tools Location |
|---|
| X | adata.layers['counts'] |
| batch | adata.obs['_scvi_batch'] |
| labels | adata.obs['_scvi_labels'] |
Summary Statistics
| Summary Stat Key | Value |
|---|
| n_batch | 1 |
| n_cells | 42729 |
| n_labels | 33 |
| n_vars | 3000 |
Training
Training data url: Not provided by uploader
If provided by the original uploader, for those interested in understanding or replicating the
training process, the code is available at the link below.
References
The Tabula Sapiens Consortium. The Tabula Sapiens: A multiple-organ, single-cell transcriptomic atlas of humans. Science, May 2022. doi:10.1126/science.abl4896
