In the software/camera folder, you can use a Princeton camera to capture fluorescence images and perform real-time unmixing, provided you install the LightField software and set the LIGHTFIELD_ROOT environment variable.
If the LIGHTFIELD_ROOT path is not set, you can run the main.exe file in the software/example folder and click Use Example and Extract Spectrum to view the example unmixing results from our paper.
⚠️ Our software supports GPU acceleration using an NVIDIA 2080Ti and CUDA 11.8.
In other environments where GPU support may be unavailable, it will fall back to CPU processing, which may result in slower performance.
Trouble Shooting
Resolving RuntimeError: Failed to resolve Python.Runtime.Loader.Initialize
If your application crashes and running main.exe via Command Prompt results in the error:
RuntimeError: Failed to resolve Python.Runtime.Loader.Initialize
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you can try unblocking Python.Runtime.dll:
Navigate to the directory containing Python.Runtime.dll.
Right-click on Python.Runtime.dll and select Properties.
In the General tab, if there's an Unblock checkbox, check it.
Click OK to apply the changes.
This issue often occurs when the .dll file is blocked by Windows after being copied from another system or downloaded. Unblocking it allows the application to access the necessary runtime components.
