ZOI VET is an application within the CarbConnect platform designed for use in veterinary microbiology. It automates the Zone of Inhibition (ZOI) measurement process for antimicrobial susceptibility testing (AST).
The application detects and measures inhibition zones from Petri dish images and determines susceptibility results as:
Sensitive (S)
Intermediate (I)
Resistant (R)
The S/I/R result is determined according to the selected bacterial strain and antibiotic.
Intended Use
ZOI VET is intended to support research workflows in veterinary microbiology laboratories by:
Automating inhibition zone detection and measurement from plate images
Standardizing AST result interpretation
Reducing manual workload
Improving consistency and result accuracy
The susceptibility interpretation uses the criteria from:
CLSI VET01S ED7:2024 Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated From Animals
Published by the Clinical and Laboratory Standards Institute (CLSI).
Limitations
This tool is strictly intended for Research Use Only (RUO).
It is not intended for clinical diagnostics, medical decision-making, or medical procedures.
For additional information, refer to the ZOI VET Learn More page on CarbConnect.
Training Data
The training dataset was generated from antimicrobial susceptibility testing (AST) performed at CarbGeM Inc. using:
Representative bacterial strains
Antimicrobial disks
Mueller-Hinton agar
Approximately 300 images of the plates were captured using various smartphone camera models to generate the training dataset.
Data Acquisition Context
The images were collected to represent real AST plate conditions in veterinary microbiology workflows. Variability in smartphone camera models was intentionally included to improve robustness across practical image capture conditions.
Output
Given an input image of a Petri dish plate, the application produces:
Detected inhibition zone measurements
Susceptibility categorization as S, I, or R
Outputs depend on the selected bacterial strain and antibiotic context.
Ethical and Regulatory Notice
This repository describes a private research tool. Availability of the repository does not imply regulatory clearance, clinical validation, or approval for diagnostic use.
Contact
For further details, please contact the CarbConnect / CarbGeM team through the appropriate internal channel or product support path.
