This dataset contains 160000 sequences of four site mutation of an enzyme active site.
The enzyme is β-subunit of tryptophan synthase (TrpB), which synthesizes L-tryptophan (Trp) from indole and L-serine (Ser).
The parent enzyme is TrpB variant Tm9D8*.
Tm9D8* differs from wildtype TmTrpB by ten amino acid substitutions (P19G, E30G, I69V, K96L, P140L, N167D, I184F, L213P, G228S, and T292S).
The 4-sitesaturation library targeted two pairs of positions: 183/184 and… See the full description on the dataset page:
https://huggingface.co/datasets/SaProtHub/Dataset-TrpB_fitness_landsacpe.
